Figure 4 | Scientific Reports

Figure 4

From: A novel fibroblast growth factor receptor 1 inhibitor protects against cartilage degradation in a murine model of osteoarthritis

Figure 4

Effects of G141 on proteoglycan loss in adult human articular cartilage.

Full thickness cartilage explants from human femur head were cultured in the absence or presence of FGF-2 (50 ng/ml) and in the presence or absence of G141 (5 μM, 10 μM) for 14 days. (A) 5 μm Paraffin sections were stained with Safranin O-fast green to identify the cartilage proteoglycan loss. Scale bar, 200 μm. (B) Culture medium were collected and the amount of GAG released into the medium were quantified by DMMB assay. GAG released into the medium was normalized as mass of GAG per milliliter (ml) of culture medium (Values are the mean ± SEM of 3 independent trials per treatment group, *p < 0.05 versus controls, #p < 0.05 versus FGF-2 alone, ##p < 0.01 versus FGF-2 alone).

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