Figure 1
ChIP-seq data reveal changes in the distribution of RXR binding in APOE3 mice brain induced by bexarotene treatment.
APOE3 mice were treated with bexarotene or vehicle as described in Materials & Methods and ChIP-seq was performed using material from cortex. (A) Venn diagram showing the number of overlapping peaks in bexarotene (Bexa) and vehicle (Veh) treated APOE3 mice (Blue: Veh, Red: Bexa); (B) Distribution of RXR binding showing significant shift towards RXR binding in promoter regions of annotated genes in bexarotene versus vehicle treated mice. (C) Most significant functional annotation categories (GO Term Biological Process, DAVID); (D) ChIP-qPCR validation data of genes with enriched RXR binding in promoter regions. Fasn gene is used as a positive control. (E) Comparative heatmap of RXR cistrome of bexarotene treated APOE3 and APOE4 mice Shown are the most significant distinct Biological Function categories identified by IPA in each experiment. Blue represents vehicle and red represents bexarotene. Gray boxes indicate no overlapping categories. Negative log10 (P value) denotes lower significance and red color higher significance.
