Figure 4: Adh is involved in the transformation of mitochondrial membrane potential and the expression of caspase-9. | Scientific Reports

Figure 4: Adh is involved in the transformation of mitochondrial membrane potential and the expression of caspase-9.

From: Adh enhances Actinobacillus pleuropneumoniae pathogenicity by binding to OR5M11 and activating p38 which induces apoptosis of PAMs and IL-8 release

Figure 4

(A) qRT-PCR results of Bcl2 in A. pleuropneumoniae 5b WT- or 5b ΔAdh-infected PAMs; (B) qRT-PCR results of Bax in A. pleuropneumoniae 5b WT- or 5b ΔAdh-infected PAMs; (C) qRT-PCR results of caspase-9 in A. pleuropneumoniae 5b WT- or 5b ΔAdh-infected PAMs; (D) The effects of a Caspase-9 inhibitor on the Adh-mediated apoptosis of PAMs; (E) Flow cytometry results of JC-1 in MAPK inhibitor-pretreated PAMs; (F) Annexin V/PI staining results of 5b WT- or 5b ΔAdh-mediated apoptosis in MAPK inhibitor-pretreated PAMs; (G) The effects of different densities of the p38 inhibitor SB203580 on 5b WT-mediated apoptosis; (H) The effects of caspase inhibitors on 5b WT- or 5b ΔAdh-mediated IL-8 release in PAMs.

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