Figure 5: Adh mediates IL-8 release through the activation of the MAPK signaling pathway.

(A) Activation of MAPK in A. pleuropneumoniae 5b WT- or 5b ΔAdh-infected PAMs; (B–E) The effects of pretreatment with SP600125, PD98059, PDTC, or SB203580 on IL-8 release in A. pleuropneumoniae 5b WT- or 5b ΔAdh-infected PAMs; (F) The effects of pretreatment with SB203580 on the expression of IL-8 mRNA in 5b WT- or 5b ΔAdh-infected PAMs; (G) The effects of pretreatment with SB203580 on the recruitment of neutrophils in 5b WT- or 5b ΔAdh-infected PAMs.