Table 2 PCR primers used for amplifying KRTAP6-n.
From: Identification of four new gene members of the KAP6 gene family in sheep
Gene | PCR primer (5′-3′) | Expected size | Annealing temp. | SSCP conditions |
|---|---|---|---|---|
KRTAP6-2 | KAP6-up1: TCTACCCGAGAACAACCTC | 429 bp | 60 °C | 10%, 9 °C, 320 V, 18 h |
KAP6-dn2: AGAGGGCATTAAAAGGCACG | ||||
KRTAP6-3 | KAP6-up1: TCTACCCGAGAACAACCTC | 399 bp | 60 °C | 9%, 3 °C, 350 V, 18 h |
KAP6-dn3: CTTCCATGATGCAGCCTAAC | ||||
KRTAP6-4 | KAP6-up2: CACCTATACTCTCCTCCATC | 358 bp | 60 °C | 10%, 7 °C, 320 V, 18 h |
KAP6-dn4: AGAAGTCTGTAGTGTTGTGC | ||||
KRTAP6-5 | KAP6-up1: TCTACCCGAGAACAACCTC | 473 bp | 60 °C | 10%, 12 °C, 300 V, 18 h |
KAP6-dn5: TAGGTTAAAAGGTAGGCTAGG |
