Figure 9
From: The BET inhibitor OTX015 reactivates latent HIV-1 through P-TEFb
OTX015 increases CDK9 recruitment to the HIV-1 LTR and induces RNAP II CTD phosphorylation.
(A) C11 cells were mock-treated or stimulated with OTX015 (0.5 μM) for 6 h. ChIP assays were performed using antibodies against CDK9, Pol II CTD-Ser2P or normal mouse IgG. PCR primers specific for the LTR promoter were used to amplify the DNA isolated from the immunoprecipitated chromatin as described in Materials and methods. (B) Each ChIP experiment was repeated three times to confirm reproducibility of the results. Real-time quantitation of the fold change relative to the DMSO control is shown. **p < 0.01.
