Figure 4

The rs6876739 genotype was associated with C7, soluble MAC and IL-1β release in recipients (A–C) and MAC activates NLRP3 inflammasomes and IL-1β release In vitro (D,E). (A,B) The C7 and soluble MAC of recipient were markedly higher in recipients of the donor rs6876739 TT genotype than in those of the donor rs6876739 CC genotype on days 7, 14, 30 after LT. (C) The IL-1β levels were markedly higher in recipients of the donor rs6876739 TT genotype than in those of the donor rs6876739 CC genotype (horizontal lines represent the median; the bottom and top of the boxes represent the 25th and 75th percentiles, respectively); (D) Western blot analysis showing that MAC stimulates NLRP3 and caspase 1 expression; (E) ELISA showing that MAC activates IL-1β release.