Figure 2 | Scientific Reports

Figure 2

From: Mycobacteria-responsive sonic hedgehog signaling mediates programmed death-ligand 1- and prostaglandin E2-induced regulatory T cell expansion

Figure 2

M. bovis BCG-induced proinflammatory responses are moderately NOTCH dependent and suppressed by SHH.

DCs were cultured with GM-CSF and IL-4 and left untreated (Med) or infected with M. bovis BCG alone or after 1 h pretreatment with the indicated inhibitors for 24 h. (mean ± SEM, n = 6–10) (a) Surface expression of maturation markers CD83, HLA-DR, CD40, CD86 and CD80 were examined by flow cytometry. Data is represented as % positive cells or MFI. (b) Cell-free supernatants from the above-said experiment were assessed for secretion of IL-6, TNF-α, IL-12p70 by cytokine bead array (mean ± SEM, n = 5). Med, Medium. *P < 0.05; **P < 0.005; ***P < 0.001 (one-way ANOVA followed by Turkey’s multiple-comparisons test).

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