Figure 8 | Scientific Reports

Figure 8

From: Mycobacteria-responsive sonic hedgehog signaling mediates programmed death-ligand 1- and prostaglandin E2-induced regulatory T cell expansion

Figure 8

Counter-regulation of NOTCH1 and SHH pathway functions during M. bovis BCG infection of DCs.

(a,b) Transcript (a) and surface (b) expression of PD-L1 in DCs infected with M. bovis BCG alone or after 1 h pretreatment of GSI by quantitative real time RT-PCR and flow cytometry (mean ± SEM, n = 7) respectively. (c–e) Immature DCs were pretreated with SHH signaling inhibitors and infected with M. bovis BCG for 6 h. NOTCH1 signaling markers, NOTCH1, JAG1 and JAG2 transcripts were analyzed using quantitative real time RT-PCR (c), NICD by immunoblotting (d) and densitometric analysis of panel D (e). (f) Model: schematic representation of the obtained results. All RT-PCR and densitometry data represents the mean ± SEM from at least 3 independent experiments and all blots are representative of 3 independent experiments. Images have been cropped for presentation; full-size blot is shown in Supplementary Fig. S2. Med, Medium. *P < 0.05; **P < 0.005; ***P < 0.001 (one-way ANOVA followed by Turkey’s multiple-comparisons test).

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