Table 1 Sequences used to generate the three synthesized HCCR-targeting shRNAs.

From: Targeting HCCR expression resensitizes gastric cancer cells to chemotherapy via down-regulating the activation of STAT3

No.

Targeting sequence

shRNA-1

5′-gatccccACAGATCTGTGCACCAAGAttcaagagaTCTTGGTGCACAGATCTGTttttt-3′

3′-gggTGTCTAGACACGTGGTTCTaagttctctAGAACCACGTGTCTAGACaaaaatcga-5′

shRNA-2

5′-gatccccTAAGATGTGAGAAGCATGGttcaagagaCCATGCTTCTCACATCTTAttttt-3′

3′-gggATTCTACACTCTTCGTACCaagttctctGGTACGAAGAGTGTAGAATaaaaatcga-5′

shRNA-3

5′-gatccccTTGTGCAGCAAGAGAGACAttcaagagaTGTCTCTCTTGCTGCACAAttttt-3′

3′-gggAACACGTCGTTCTCTCTGTaagttctctACAGAGAGAACGACGTGTTaaaaatcga-5′

  1. All of these sequences were sliced by the Bgl II and Hind III enzymes and inserted into pSuper vectors.
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