Figure 3
The effects of the interaction of XIAP with procaspase-9 on the transnitrosylation from procaspase-9 to XIAP and the cleavage of procaspase-9 induced by I/R.
The time course of interaction was detected using immunoprecipitation. (A) Sample proteins were immunoprecipitated (IP) with anti-XIAP antibody followed by immunoblot (IB) with anti-procaspase-9 antibody and IP with anti- procaspase-9 antibody, followed by IB with anti-XIAP antibody. Bands were quantified and the intensities were represented as fold-increase vs. the sham group. Data were the means ± S.D. from four independent experiments (n = 4). *P < 0.05 vs. the sham group. (B) Rats were treated with TAT-AVPY and TAT-MMLL. Sample proteins were immunoprecipitated (IP) with anti-XIAP antibody, followed by immunoblot (IB) with anti-procaspase-9 antibody. (C) Rats were treated with TAT-AVPY and TAT-MMLL to observe their effects on the S-nitrosylation of XIAP and procaspase-9 and the cleavage of procaspase-9 (cleaved caspase-9). Bands were quantified and the intensities presented as fold-increase vs. the sham group. Data were the means ± S.D. from four independent experiments (n = 4). *P < 0.05 vs. the sham group, #P < 0.05 vs. the R3 group.
