Figure 2: NL1-R induces presynaptic differentiation independently of SLB membrane.
From: Robust Type-specific Hemisynapses Induced by Artificial Dendrites
(a–c) Immunocytochemistry (ICC) analysis for synaptic marker proteins in cultured hippocampal neuron. (a) NL1-R has the strongest synaptogenic activity both on SLB membrane beads and SAV Dynabeads when incubated with DIV14 hippocampal neuron culture for 2 d. (b) NL1-R shows the most prominent synaptogenic activity when immobilized on microbeads without SLB membrane. Synapsin I (Syn I) and bassoon (Bsn) aggregation were induced by PDK-coated silica beads, NL1-R on SLB, and NL1-R on SAV Dynabeads. MFI = mean fluorescence intensity. Scale bars = 10 μm. ∗∗∗p < 0.001.
