Figure 6: Excitatory hemisynapses release glutamate neurotransmitter.
From: Robust Type-specific Hemisynapses Induced by Artificial Dendrites
(a) Schematics of the measurement of glutamate spillover using engineered glutamate sensor iESFb. (b) NL1-R artificial dendrites were added to the axonal compartment of DIV14 cells. After incubation for 2 d, the iESFb beads were spread to the same side. Scale bar = 10 μm. (c) The neurons were stimulated by potassium shock on the somal compartment. When compared with bare SAV beads, the iESFb beads near the artificial dendrites exhibited enhanced fluorescence due to the glutamate spillover.
