Figure 9
From: Meiotic chromosome mobility in fission yeast is resistant to environmental stress
The actin cytoskeleton of horsetail cells.
Actin is stained in red, tubulin in green, DNA in blue (DAPI). Maximum projections of image stacks shown. (A, a) Non-irradiated horsetail cell displaying a normal actin cytoskeleton characterized by numerous cortical patches that are of similar size and distributed throughout the cell. Irradiation with 200 Gy leads to an aggregation of the actin patches (A, b–d). (B) The percentage of cells with disordered actin distribution increases with time after 200 Gy X IR. (C) Actin (red) and DNA (blue) distribution in control (a) and ascospores formed after 200 Gy irradiation of horsetail cells (b–d). (b) IR-induced DNA fragmentation leads to DAPI bodies (light blue) outside spores (arrow). (c) The actin cytoskeleton fails to form patches and is sometimes absent in spores that lack DNA (arrow). (d) Ascus showing severely damaged nuclei, spores and precipitated actin (red dot). Bar: 4 μm. (D) Irradiation in the horsetail stage induces defective ascospores. Percentage of ascospores investigated (>150 from 3 different experiments; ±SD).
