Figure 2: Biological characterizations of human dental follicle cells (hDFCs) from cleidocranial dysplasia (CCD) patient and control Individuals. | Scientific Reports

Figure 2: Biological characterizations of human dental follicle cells (hDFCs) from cleidocranial dysplasia (CCD) patient and control Individuals.

From: RUNX2 Mutation Impairs 1α,25-Dihydroxyvitamin D3 mediated Osteoclastogenesis in Dental Follicle Cells

Figure 2

(a) Representative photomicrographs of hDFCs from normal control and CCD patient, RUNX2+/+, control individuals; RUNX2+/m, CCD patient (n = 6). Scale bar = 500 μm. (b) Immunohistochemical staining of vimentin and keratin for CCD hDFCs (n = 3). Scale bar = 100 μm. (c) The RUNX2 mRNA levels of control and CCD hDFCs were detected by Quantitative real-time polymerase chain reaction (qRT-PCR) after cultured for 3 d (n = 3). (d) Immunofluorescence assay of RUNX2 for control hDFCs (upper panel) and CCD hDFCs (middle panel) (n = 3). Negative controls (lower panel) were incubated with goat IgG instead of anti-RUNX2 as first antibody. Scale bar = 20 μm. (e) MTT proliferation assay for control hDFCs and CCD hDFCs at different time points treated with 10−7 M 1α,25-(OH)2D3 or equal volume of ethanol (n = 4). *P < 0.05. **P < 0.01. ***P < 0.001.

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