Figure 5: 1α,25-(OH)2D3 strongly facilitated osteoclast formation induced by RUNX2+/+ hDFCs but not by RUNX2+/m hDFCs. | Scientific Reports

Figure 5: 1α,25-(OH)2D3 strongly facilitated osteoclast formation induced by RUNX2+/+ hDFCs but not by RUNX2+/m hDFCs.

From: RUNX2 Mutation Impairs 1α,25-Dihydroxyvitamin D3 mediated Osteoclastogenesis in Dental Follicle Cells

Figure 5

hDFCs from normal control (RUNX2+/+) and CCD patient (RUNX2+/m) were co-cultured with PBMCs in culture medium containing 1 × 10−7 M 1α,25-(OH)2D3 or equal volume of ethanol for 16 d or 21 d. (a) TRAP straining of the co-cultures of PBMCs and RUNX2+/+ or RUNX2+/m hDFCs in the stimulation of 1α, 25-(OH)2D3 on 16 d (n = 4). Scale bar = 100 μm. (b) The quantification of TRAP straining for PBMCs and hDFCs co-cultures. Eight sites on each coverslip were measured for the number of TRAP+ multinucleated cells (≥3 nuclei per cell) and TRAP+mononuclear / binuclear cells on 16 d (n = 4). (c) Scanning electron microscopy (SEM) analyses for hDFCs and PBMCs co-cultures. hDFCs and PBMCs were co-cultured on bovine cortical bone slices (6 × 6 mm2) for 21 d with 1 × 10−7 M 1α,25-(OH)2D3 . SEM was used to visualize resorption lacunae on bone slices (n = 3). **P < 0.01. ***P < 0.001.

Back to article page