Table 1 ELISA screening of candidate peptides identified by NGPD as being enriched against IgG from S. Typhimurium infected pigs.

From: Mapping polyclonal antibody responses to bacterial infection using next generation phage display

Peptide sequencea

In silico assessmentb

ELISA assessment

AUC of ROCc

P valuec

AEGEFGFTNAIELA

10/12

0.965

<0.001

AEGEFTPQTHSQQP

7/12

0.868

<0.001

EGEFCSRAI*SQALCG

7/12

0.667

0.048

AEGEFSQTSLSAAT

6/12

0.922

<0.001

AEGEFDPKRGRHMV

6/12

0.762

0.002

AEGEFFDARNHPDE

6/12

0.852

<0.001

EFCPLEKRISFDVRG

5/12

0.743

0.004

EGEFCPLKTYQL*CAG

5/12

0.710

0.013

AEGEF*TTP*SPSQ

5/12

0.760

0.002

GEFCDGPNSSSIAAG

5/12

0.832

<0.001

AEGEFKSNAFP*AL

5/12

0.832

<0.001

AEGEFP*SPKMRVW

5/12

0.637

0.103

EGEFCTHCKFALL*CG

3/12

0.619

0.158

AEGEFAPQTHSQQP

3/12

0.880

<0.001

EGEFCTCAQSLRG*CG

3/12

0.569

0.409

AEGEFQ*QKQVATP

3/12

0.946

<0.001

AEGEFPNLNGTAAT

3/12

0.948

<0.001

AEGEFPLHNGNERL

2/12

0.955

<0.001

AEGEFAKCRASKRQ

2/12

0.632

0.117

AEGEFATSKK*QAQ

2/12

0.908

<0.001

GEFCEVLQSWYQFCG

2/12

0.693

0.022

AEGEFR*QTSWLIA

2/12

0.676

0.036

EFCCTPPGKPCEVRG

2/12

0.619

0.155

AEGEFCP*NVLA*CAG

2/12

0.750

0.003

AEGEFHYSVSTPGT

2/12

0.887

<0.001

AEGEFLATVASN*M

2/12

0.899

<0.001

AEGEFVQATDTN*S

2/12

0.885

<0.001

Control peptide

–

0.581

0.338

  1. aOpal and ochre codons are denoted by an asterisk. Residues coded for by these stop codons were omitted in the synthesised peptides. N-terminal residues AEGEF are phage coat protein residues. Constrained peptides also contain a phage coat protein G residue at their C-terminus.
  2. bNumber of IgG samples that the peptides was enriched against/total number of IgG samples: enrichment was defined using a Z score of ≥2.0.
  3. cROC curves were produced for the recognition of peptides with sera from infected (n = 24) and non-infected (n = 24) pigs in an ELISA. AUC and corresponding p values are listed.
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