Table 2 ELISA screening of candidate peptides identified by NGPD as being enriched against IgY from chickens infected with the serovar S. Enteritidis.

From: Mapping polyclonal antibody responses to bacterial infection using next generation phage display

Peptide sequencea

In silico assessmentb

ELISA assessment

AUC of ROCc

P valuec

AEGEFRESPQNSLA

2/9

0.69

0.030

AEGEFLPRPIQPTP

2/9

0.60

0.260

AEGEFAPQGPAPTA

2/9

0.68

0.045

AEGEFPRNQEHFAM

2/9

0.60

0.260

AEGEFRSQSRRFHL

2/9

0.64

0.127

AEGEFEPQQSARPS

2/9

0.77

0.002

AEGEFAPHMQFKTS

2/9

0.67

0.050

AEGEFWARTHVQKV

2/9

0.67

0.059

CSTRRPIQTTCG

2/9

0.76

0.003

AEGEFFVNRALINQ

2/9

0.83

<0.001

AEGEFAPSRQHRSW

2/9

0.71

0.021

AEGEFQGASGRSCR

2/9

0.54

0.661

AEGEFMALLNQSYP

2/9

0.67

0.063

CQPLQSINITCG

2/9

0.79

0.001

AEGEFQALPKREPP

2/9

0.69

0.034

Control peptide

 

0.54

0.696

  1. aFor linear peptides, N-terminal residues AEGEF are phage coat protein residues. Constrained peptides contain a phage coat protein G residue at their C-terminus.
  2. bNumber of IgY samples that the peptides was enriched against/total number of IgY samples: enrichment was defined as peptides giving a Z score of ≥2.0.
  3. cROC curves were produced for the recognition of the peptides with IgY from S. Enteritidis infected (n = 19) and S. Typhimurium or S. Hadar infected (n = 25) chickens in an ELISA. AUC and corresponding p values are listed.
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