Figure 7: Effects of LPM580153 on inhibitory effects of corticosterone on levels of p-CREB, BDNF, p-ERK, p-AKT, and p-mTOR.
From: Antidepressant-like Effects of LPM580153, A Novel Potent Triple Reuptake Inhibitor
SH-SY5Y cells were exposed to corticosterone (300 μM) in absence or presence of LPM580153 for 24 h, and the cells were lysate. The total protein was separated by SDS-PAGE gel under the same condition, and then the gel was cropped based on molecular weight of the target protein. The expression of the tested protein CREB/p-CREB and BDNF (A), as well as the ERK/p-ERK, AKT/p-AKT, mTOR/p-mTOR (B) were analyzed by immunoblotting using specific antibody, in which the density was semi-quantified and presented (C). ###p < 0.01, compared with the control group; ***p < 0.01, compared with the model group.
