Table 1 List of plasmids and strains used in this study.
| Â | Description | Reference |
|---|---|---|
Plasmids | ||
 pYY0040 pET-AACP | Deletion of both His-Tag gene and Nus-Tag gene from pET44a tcsA-ACP cloned as NdeI/HindIII fragment into pET28a | |
 pYY0081 | stw ACP cloned as NdeI/HindIII fragment into pET28a | This study |
 pYY0082 | sts FAS ACP cloned as NdeI/HindIII fragment into pET28a | This study |
 pYY0098 | fkbP-PCP cloned as NdeI/HindIII fragment into pET28a | This study |
 pYY0072 | FKPPT1 cloned as a NdeI/HindIII fragment into pET44a | This study |
 pYY0078 | FKPPT2 cloned as a NdeI/HindIII fragment into pET44a | This study |
 pYY0073 | FKPPT3 cloned as a NdeI/HindIII fragment into pET44a | This study |
 pYY0077 | FKPPT4 cloned as a NdeI/HindIII fragment into pET44a | This study |
 pYY0074 | FKACPS cloned as a NdeI/HindIII fragment into pET44a | This study |
 pYY0099 | FKPPT1 cloned as a NdeI/NotI fragment into pIJ8660 | This study |
 pYY0100 | FKPPT2 cloned as a NdeI/NotI fragment into pIJ8660 | This study |
 pYY0101 | FKPPT3 cloned as a NdeI/NotI fragment into pIJ8660 | This study |
 pYY0102 | FKPPT4 cloned as a NdeI/NotI fragment into pIJ8660 | This study |
Strains | ||
 S. tsukubaensis L19 | An industrial FK506 producing strain | This study |
 sHJ0015 | Deletion of FKPPT1 in S. tsukubaensis L19 | This study |
 sHJ0016 | Deletion of FKPPT2 in S. tsukubaensis L19 | This study |
 sHJ0017 | Deletion of FKPPT3 in S. tsukubaensis L19 | This study |
 sHJ0018 | Deletion of FKPPT4 in S. tsukubaensis L19 | This study |
 sHJ0019 | Overexpression of FKPPT1 in S. tsukubaensis L19 | This study |
 sHJ0020 | Overexpression of FKPPT2 in S. tsukubaensis L19 | This study |
 sHJ0021 | Overexpression of FKPPT3 in S. tsukubaensis L19 | This study |
 sHJ0022 | Overexpression of FKPPT4 in S. tsukubaensis L19 | This study |
