Figure 5: Morphological phenotypes of hMSCs.

(a–d) Plot of circularity γ vs. aspect ratio AR for Type A–Type D hMSCs at t = 20 d. Representative fluorescence images (actin: green, nucleus: blue) are presented in insets. Each data set represents from n > 30 cells. Morphological populations were grouped by two ellipses characteristic for Type A hMSCs (89%) and Type B hMSCs (100%). Type A phenotype can represent 70% of Type C hMSCs, while Type B phenotype represents 93% of Type D hMSCs.