Figure 3 | Scientific Reports

Figure 3

From: Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: The influence of tissue source and inflammatory stimulus

Figure 3

MSC immunoprofile (via flow cytometry) and trilineage differentiation of FP and SF-MSCs.

(a) Both FP and SF-MSC populations were immunopositive for CD73 (98.8%), CD90 (98.3%), CD105 (99.6%); FP-MSCs showed significantly more cells which were positive for the cocktail of ISCT defined negative MSC selection markers compared to SF-MSCs (P = 0.046). (b) Single antibody cytometric analysis revealed a high positivity for CD34 in FP-MSCs (n = 3), but no positivity for CD11b, CD19, CD45 and HLA-DR. Error bars indicate SEM. (c) Cell positivity for CD14 was variable between donors and matched cell populations (n = 5). (d) The two MSC populations demonstrate differentiation potential when grown in different media; Chondrogenesis is shown by GAG production (purple metachromasia when stained with toluidine blue), adipogenesis is shown by the formation of lipid vesicles (oil red O) and osteogenesis is shown by the activity of alkaline phosphatase (red staining). Images are representative of the differentiation potential of all six donor matched samples. Scale bars represent 100 μm.

Back to article page