Figure 4

Immunogenic properties of FP and SF-MSCs after stimulation with IFN-γ (error bars indicate SEM).

(a) The production of co-stimulatory markers (CD40, CD80 and CD86) and the class II MHC receptor HLA-DR for FP-MSCs is shown after 48 hours of stimulation with IFN-γ along with unstimulated controls. FP-MSCs do not produce co-stimulatory molecules regardless of stimulation, but show greater HLA-DR positivity at 25 ng/ml (39.8% ± 14.3, p < 0.0001) and at 500 ng/mL (46.9% ± 14.9, p < 0.0001) stimulation with IFN-γ. (b) SF-MSCs show no positivity for co-stimulatory marker, even when stimulated, but produce significantly higher levels of HLA-DR at 25 ng/ml (13.1% ± 6.4, P = 0.0015) and at 500 ng/mL IFN-γ (9.8% ± 4.3, P = 0.025). (c–d) Donor variability in the levels of induced HLA-DR on FP-MSCs and SF-MSCs was observed at both 25 ng/mL (C) and 500 ng/ml (D). However, donor number 2 (indicated by the square data points) had higher positivity for HLA-DR compared to other donors for both FP-MSCs and SF-MSCs and for both of the IFN-γ doses tested.