Figure 1: Production of superoxide and conidia of Coniothyrium minitans CmNox1 and CmNox2 mutants. | Scientific Reports

Figure 1: Production of superoxide and conidia of Coniothyrium minitans CmNox1 and CmNox2 mutants.

From: Nox Complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans

Figure 1

(a) Detection of superoxide using colony staining with NBT solution (0.05% w/v, 30 min) of cultures of C. minitans wild-type strain ZS-1, CmNox1 deletion mutant ΔCmNox1-6, CmNox2 deletion mutant ΔCmNox2-323 on PDA. (b) Pycnidial and conidial production of C. minitans wild-type strain ZS-1, CmNox1 deletion mutant ΔCmNox1-6, complementary transformant CmNox1-C8, and CmNox2 deletion mutant ΔCmNox2-323 12 days after incubation on PDA. Compared to ZS-1, CmNox1-C8 and ΔCmNox2-323, ΔCmNox1-6 lost the ability to produce pycnidia.

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