Figure 3: NADPH oxidase complexes in C. minitans. | Scientific Reports

Figure 3: NADPH oxidase complexes in C. minitans.

From: Nox Complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans

Figure 3

Expression analysis of CmNox1 (a) and CmNox2 (b). Total RNA was isolated from mycelia of ZS-1 cultured alone or dual-incubated with S. sclerotiorum on PDA, and used for cDNA synthesis. The transcript level of CmActin was used to normalize different samples. Bars represent means and standard deviations (three replications). (c) Schematic drawing showing the NADPH oxidase complexes in C. minitans. (d) Yeast two-hybrid assay of interaction between CmNoxR, CmBem1, and CmRac1. (e) Colony morphology of CmRac1 deletion mutant ΔCmRac1-79, CmNoxR deletion mutant ΔCmNoxR-107 and CmBem1 deletion mutant ΔCmBem1-344 grown on PDA at 20 °C for 12 days.

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