Figure 4: Interaction of CmNox1 with CmSlt2.

(a) Yeast two-hybrid assay of interaction between CmNox1 and CmSlt2. (b) Co-IP assays. Western blots of total proteins and proteins eluted from anti-Flag agarose from transformant (CmSlt2-GFP and vector Flag) and transformant (CmSlt2-GFP and CmNox1-Flag) were detected with anti-Flag or anti-GFP antibodies. (c) Bimolecular fluorescence complementation (BiFC) analysis of the interaction between CmNox1 and CmSlt2 tagged with YFP in Nicotiana benthamiana.