Figure 1: IL-20 was involved in osteoblastogenesis during bone fracture healing in vivo. | Scientific Reports

Figure 1: IL-20 was involved in osteoblastogenesis during bone fracture healing in vivo.

From: Anti-IL-20 monoclonal antibody promotes bone fracture healing through regulating IL-20-mediated osteoblastogenesis

Figure 1

(a) Levels of IL-20 in serum from mice with bone fracture were analyzed at the indicated time during fracture healing. Values are means ± SD of 6 mice. Data are representative of three independent experiments. *P < 0.05 versus 0 day post-fracture. (b) Representative figures of micro-CT analyses of the fracture callus of mice 21 and 42 days post-fracture, followed by no treatment (Control), 3 mg mIgG/kg/3 d treatment, and 3 mg 7E/kg/3 d treatment (n = 8/group). Images of these individual slices were taken from the central region of each callus. Data are representative of three independent experiments. (c) BMD at the fracture callus was assessed using micro-CT scans 21 and 42 days post-fracture (n = 8/group). Values are means ± SD. Data are representative of three independent experiments. *P < 0.05 versus mIgG controls. (d) ALP staining analysis and quantification of the number of osteoblasts in fracture callus 21 days post-fracture (n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. *P < 0.05 versus mIgG controls. (e) TRAP staining analysis and quantification of the number of osteoclasts in bone surface 21 days post-fracture (n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. *P < 0.05 versus mIgG controls.

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