Figure 2: IL-20R1 deficiency increased osteoblast differentiation and promoted fracture healing by increasing BMD in the mouse fracture model.
(a) Levels of IL-20 in serum from IL-20R1+/+ and IL-20R1−/− mice with bone fracture were analyzed at the indicated times during fracture healing. Values are means ± SD of 6 mice. Data are representative of three independent experiments. *P < 0.05 versus IL-20R1+/+ mice 0 day post-fracture. #P < 0.05 versus IL-20R1−/− mice 0 day post-fracture.♮P < 0.05 versus IL-20R1−/− mice post-fracture. (b) Radiographs of fractured right femurs from IL-20R1+/+ and IL-20R1−/− mice (n = 8/group). The radiographic time course is shown at the callus formation for the mice in each group. Data are representative of three independent experiments. (c) Radiological assessments on fracture healing by fracture healing stage score of the X-ray images from IL-20R1+/+ and IL-20R1−/− mice (n = 8/group). Data are representative of three independent experiments. (d) Representative figures of micro-CT analyses of the fracture callus of IL-20R1+/+ and IL-20R1−/− mice 21 and 42 days post-fracture (n = 8/group). Images of these individual slices were taken from the central region of each callus. Data are representative of three independent experiments. (e) BMD at the fracture line was assessed using micro-CT scans 21 and 42 days post-fracture (n = 8/group). Values are means ± SD. Data are representative of three independent experiments. *P < 0.05 versus IL-20R1+/+ mice. (f) ALP staining analysis and quantification of the number of osteoblasts in the fracture callus 21 days post-fracture (n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. *P < 0.05 versus IL-20R1+/+ mice. (g) TRAP staining analysis and quantification of the number of osteoclasts in bone surface 21 days post-fracture (n = 8/group). Values are means ± SD of 3 frozen sections. Data are representative of three independent experiments. *P < 0.05 versus IL-20R1+/+ mice.
