Figure 7: IL-20R1 deficiency enhanced osteoblastogenic gene expression.

Primary mouse preosteoblastic calvaria cells were isolated from 24-h-old IL-20R1^+/+ and IL-20R1^−/− mice and cultured under osteogenic conditions for 21 days. mRNA was isolated, and (a) RUNX2 and (b) Atf4 transcripts were analyzed using RTQ-PCR with specific primers. All experiments were run three times, with similar results. Data are from a representative experiment. (c) Mature osteoblasts derived from IL-20R1^+/+ and IL-20R1^−/− mice were incubated with IL-20 (200 ng/ml) for 6 h. mRNA was isolated and sclerostin transcripts were analyzed using RTQ-PCR with specific primers. *P < 0.05 versus IL-20R1^+/+ untreated cells. All experiments were run three times, with similar results. Data are from a representative experiment.