Figure 5: The uptake of exogenous CnB is CD14-dependent. | Scientific Reports

Figure 5: The uptake of exogenous CnB is CD14-dependent.

From: Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4

Figure 5

(a) Co-localization of exogenous CnB and CD14. The CD14-cherry-transfected Hek293 cells were co-incubated with CnB-GFP for 30 min, fixed, and visualized using a confocal laser scanning microscope (63×, scale bar 10 μm). (b,c) Influence of CD14 knock down on CnB uptake. The influence of CD14 knock down on CnB uptake was analysed by western blot analysis (b) or FI (c). (d) The binding of CnB to sCD14 or sCD14 to CnB. To black ELISA plates, 10 μg/ml CnB or sCD14 was immobilized, and CnB-GFP or DyLight 488-labeled CD14 were incubated with the corresponding ELISA plates. Data represent mean ± s.e.m. from three independent experiments. *P < 0.05 (t-test, two-tailed).

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