Figure 4
From: Engineering formation of multiple recombinant Eut protein nanocompartments in E. coli
Higher order structures formed upon expression of EutJ, EutP and EutQ in E. coli.
Subcellular structures formed in E. coli C2566 observed by thin sectioning and TEM. Images are as follows: E. coli C2566 cells expressing (A) empty plasmid as a control, (B) EutS, (C) EutJ, (D) EutP, (E) EutQ, (F) EutJS, (G) EutPS, (H) EutQS, (I) EutSMNLK, (J) EutJSMNLK, (K) EutPSMNLK and (L) EutQSMNLK. Bold arrowheads indicate BMC-like structures, also highlighted with a circle; dashed arrowheads indicate other higher order structures. All images were taken at a magnification of x 53,000. The scale bar represents 100 nm. Representative crops of images are displayed, additional images are provided in Supplementary Figures 7–9.
