Figure 5
From: Engineering formation of multiple recombinant Eut protein nanocompartments in E. coli
Isolated native Eut BMCs and recombinant nanocompartments.
SDS-PAGE analysis of isolated native Eut BMCs from S. enterica LT2 and recombinant nanocompartments from E. coli C2566 cells expressing EutSMNLK, EutQSMNLK or EutQ. Corresponding TEM images of isolated and negatively stained polyhedral bodies are shown underneath. All samples were prepared using the centrifugation protocol described in the methods78. (A) Native Eut BMCs isolated from S. enterica LT2 grown on ethanolamine. (B) Recombinant EutSMNLK nanocompartments isolated from E. coli C2566. (C) Recombinant EutQSMNLK nanocompartments isolated from E. coli C2566. (D) Negative control, EutQ expressed in E. coli C2566. Bands labeled with asterisks were excised from the gel and protein identities were confirmed by LC/MS, those labeled with a question mark had peptide coverage of less than 50%. Predicted molecular weights of proteins are: EutS (11.6 kDa), EutM (9.8 kDa), EutN (10.4 kDa), EutK (17.5 kDa), EutL (22.7 kDa), EutQ (25.0 kDa). The scale bar on TEM images represents 100 nm.
