Figure 3
Vimentin binds to keratin via its YRKLLEGEE region located at the end of the coil 2B domain.
(A) Schematic representation of the coil 2B domain of Vim showing the modified amino acids by site-directed mutagenesis. (B) Vim mRNA expression by qRT-PCR analysis from epidermal cultures that were transiently expressing Wt Vim or its mutants, at 48 h after transfection (C) Diameter of keratinocyte colonies from parallel cultures as in (B). Data is the mean of duplicate experiments with n = 16 ± SD *p ≤ 0.05. Note that mutated Vim decreased the size of keratinocyte colonies. (D) Immunoblot assays of the Vim proteins that were co-immunoprecipitated with KRT14 at 48 h post-transfection.
