Figure 5
Decreased migration of keratinocytes electroporated with plasmid harboring Vimentin mutants in the –YRKLLEGEE- sequence of the Vimentin gene.
Keratinocytes were electroporated with a Wt Vim or its mutants and they were seeded in a MRMA as above to measure cell migration. We seeded MRMA epidermal cultures for 24 h and thereafter treated them for 6 h with EGF, with or without the plasmid harboring the Vim genes. Then, after photographing the cultures under phase contrast microscopy, we determined the distance of migration by quantifying the area of the MRMA and then calculated its radius. From these data, distance of migration was calculated. (A) Representative images of MRMA assay at 6 h. (B) Graph showing the distance of migration under each treatment. Note that cultures treated with the plasmid harboring the mutant Vim genes had a reduced distance of migration in comparison to control cultures. Data is the mean of duplicate experiments with n =16 each, ± SD *p ≤ 0.05.
