Figure 4: Osteogenic and adipogenic induction of hESC-derived perivascular progenitors and primary perivascular cells.
From: A novel lineage restricted, pericyte-like cell line isolated from human embryonic stem cells
(a) All cells were cultured in either growth media (top row) or osteogenic induction media (bottom row). Cells were fixed and stained with Alizarin Red S to qualify the extent of calcium deposits at day 21 of culture. Qualitatively, extensive Alizarin Red S suggests that 017-PC-O cells, Pl-PCs and MSCs have osteogenic differentiation potential. (b) All cells were cultured in either growth media (top row) or adipogenic induction media (bottom row). Cells were fixed and stained with Oil Red O to assess lipid accumulation at day 14 of culture. Only MSCs stained positive for Oil Red O and have adipogenic differentiation potential.
