Figure 3
In vivo pro-angiogenic properties of Gelfoam (A), decellularized (B), genipin-treated (C) and glutaraldehyde-treated tracheal matrices (D). Representative examples of chicken chorioallantoic membrane (CAM) implanted with fragments of Gelfoam, decellularized, genipin-treated and glutaraldehyde-treated tracheal matrices. Samples were placed on the CAM surface of 8-day-old embryos and photographed 4 days later. The samples induced a “spoke-wheel” pattern of new vessels. The scale bar is 1 mm. (E) Effect of Gelfoam, decellularized, genipin-treated and glutaraldehyde-treated tracheal matrices on the number of converging blood vessels 4 days post-implantation. Genipin cross-linking induces a significant (p < 0.05) increase, comparable to the control, in the number of newlybuilt blood vessels. *p < 0.05 with respect to negative control; #p < 0.05 with respect to glutaraldehyde-treated tracheal matrix.
