Figure 2 | Scientific Reports

Figure 2

From: Reduced expression of cytochrome oxidases largely explains cAMP inhibition of aerobic growth in Shewanella oneidensis

Figure 2

S. oneidensis CpdA is a cAMP phosphodiesterase.

(A) Complementation of growth defect of the cpdA mutant in LB broth with the wild-type as control (WT). S. oneidensis cpdA (SocpdA) and E. coli cpdA (EccpdA) were placed behind the IPTG-inducible Ptac promoter as described in the experimental procedures. The cpdA mutants carrying empty vector (vec), S. oneidensis cpdA (SocpdA) and E. coli cpdA (EccpdA) were examined without IPTG or with 0.2 mM IPTG. (B) cAMP levels in cultures in (A) The averaged cAMP level in WT was set to 100%, to which cAMP levels in other strains were normalized. Experiments were performed at least three times with error bars representing the standard deviation.

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