Table 1 Strains and plasmids used in this study.
Strain or plasmid | Description | Source or reference |
|---|---|---|
E. coli strain | ||
DH5α | Host strain for plasmids | Lab stock |
WM3064 | Donor strain for conjugation; ΔdapA | W. Metcalf, UIUC |
S. oneidensis strain | ||
MR-1 | Wild type | Lab stock |
HG0266 | ΔccmF derived from MR-1 | 26 |
HG0624 | Δcrp derived from MR-1 | 11 |
HG2364 | ΔccoN derived from MR-1 | 17 |
HG3901 | ΔcpdA derived from MR-1 | This study |
HGCYA | Δcya (ΔcyaAΔcyaBΔcyaC) derived from MR-1 | This study |
HGCCO | Δcco (ΔccoNOPQ) derived from MR-1 | 8 |
HGCYD | Δcyd (ΔcydABX) derived from MR-1 | 21 |
HG3901-0266 | ΔcpdAΔccmF derived from MR-1 | This study |
HG3901-0624 | ΔcpdAΔcrp derived from MR-1 | This study |
HG3901-CYA | ΔcpdAΔcya derived from MR-1 | This study |
HG3901-CCO | ΔcpdAΔcco derived from MR-1 | This study |
HG3901-CYD | ΔcpdAΔcyd derived from MR-1 | This study |
Plamid | ||
pHGM01 | Apr Gmr Cmr suicide vector | 26 |
pHGE-Ptac | IPTG-inducible Ptac expression vector | 28 |
pHGEI01 | Integrative lacZ reporter vector | 33 |
pHGE-Ptac-cpdA | Vector for expressing cpdA | This study |
pHGE-Ptac-EccpdA | Vector for expressing E. coli cpdA | This study |
pHGE-Ptac-hemA | Vector for expressing hemA | This study |
pHGE-Ptac-ccmF | Vector for expressing ccmF | This study |
pHGE-Ptac-cyd | Vector for expressing cydABX | 8 |
pHGEI01-cpdA | Vector for measuring cpdA expression | This study |
pHGEI01-hemA | Vector for measuring hemA expression | This study |
pHGEI01-ccmF | Vector for measuring ccmF expression | This study |
pHGEI01-cco | Vector for measuring cco expression | This study |
pHGEI01-cyd | Vector for measuring cyd expression | This study |
pHGEI01-hemG2 | Vector for measuring hemG2 expression | This study |
pHGEI01-hemC | Vector for measuring hemC expression | This study |
pHGEI01-ccmA | Vector for measuring ccmA expression | This study |
