Figure 7
pDCs enhance IMQ-induced inflammation and T-cell responses.
The C57BL/6-background WT mice (n = 6), Siglechkd mice (n = 6) and pDC-ablated mice (n = 6) were treated topically with IMQ cream on the shaved back every other day for 6 days. (a) Serum production of cytokines was measured 6 days after treatment with IMQ cream by ELISA. Data are the mean ± s.d. from six individual samples in a single experiment. *P < 0.01 compared with WT mice. (b) The frequency of BrdU+ lymphocyes in skin-draining LNs was analyzed 6 days after treatment with IMQ cream by flow cytometry. Data are presented as a dot plot and numbers represent the proportion of positive cells in each quadrant. (c) The constituency of T-cell subsets in skin-draining LNs (left panel) and their intracellular production of IFN-γ and IL-17 (right panel) were analyzed 6 days after treatment with IMQ cream by flow cytometry. Data are presented as a dot plot and numbers represent the proportion of positive cells in each quadrant. (d) The expression of FasL and cell surface molecules on leukocytes in skin-draining LNs was analyzed 6 days after treatment with IMQ cream by flow cytometry. Data are presented as a dot plot and numbers represent the proportion of positive cells in each quadrant. All data are representative of at least three independent experiments.
