Figure 1
From: Hsp90aa1: a novel target gene of miR-1 in cardiac ischemia/reperfusion injury
MicroRNA-1 (miR-1) expression in the myocardium of a rat model of ischemia/reperfusion (I/R).
(A) Schematic outline of I/R surgery and experimental groups. (B) MiR-1 expression in rat myocardium by qRT-PCR assay. (C) The apoptosis of cardiomyocytes by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptotic cells are stained in red. The scale bar is 100 μm. (D) Hsp90, Bax and Bcl-2 mRNA expression in rat myocardium by quantitative reverse transcription-PCR (qRT-PCR) assay. (E) Hsp90, Bax and Bcl-2 protein expression in rat myocardium by Western blot assay. *p < 0.05, **p < 0.01, ***p < 0.001. N = 5–8.
