Figure 2
From: Hsp90aa1: a novel target gene of miR-1 in cardiac ischemia/reperfusion injury
Apoptosis associated genes expression in cardiomyocytes after enforced expression of microRNA-1 (miR-1) mimic or miR-1 inhibitor.
(A) MiR-1 level in NRVCs was detected by qRT-PCR assay. (B) Quantitative analysis of the shifts of mitochondrial potentials in NRVCs with transfection of miR-1 mimic or miR-1 inhibitor. An increase in the bar indicates a shift in the fluorescence ratio correlating with an increase in mitochondrial depolarization. (C) Bax, Bcl-2 and caspase-3 protein expression were detected by Western blot assay. *p < 0.05, **p < 0.01, ***p < 0.001. N = 3.
