Figure 4
From: Hsp90aa1: a novel target gene of miR-1 in cardiac ischemia/reperfusion injury
MicroRNA-1 (miR-1) inhibits Hsp90aa1 expression, contributing to apoptosis of cardiomyocytes under conditions of OGD.
(A) Expression of Hsp90aa1 in NRVCs was detected by Western blot assay. (B) Expressions of Bax, Bcl-2 and active caspase-3 in OGD-treated NRVCs with over-expression of Hsp90aa1 by Western blot assay. (C) MiR-1 level in NRVCs with transfection of miR-1 mimic or Hsp90aa1 siRNA was detected by qRT-PCR assay. (D) Apoptosis of NRVCs by TUNEL assay. (E) Expressions of Hsp90aa1, Bax, Bcl-2 and active caspase-3 in OGD-treated NRVCs with transfection of miR-1 mimic and Hsp90aa1 siRNA, respectively. The scale bar is 100 μm. Data are shown as mean ± Standard Deviation, *p < 0.05, **p < 0.01. N = 3.
