Figure 1: Effect of the cationic polymers on the phenotype of tumour-infiltrating MDSCs in vitro. | Scientific Reports

Figure 1: Effect of the cationic polymers on the phenotype of tumour-infiltrating MDSCs in vitro.

From: Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

Figure 1

(A–C) The concentrations of IL-12, TNF-α, IL-10 and TGF-β in the supernatant of sorted MDSCs which were (A) treated with C-dextran, PEI or dextran (25 μg mL−1) for 6 hours; (B) pre-treated with PBS, 20 μg mL−1 TLR-4-neutralising antibody (MTS510) or 20 μg mL−1 control IgG (Rat IgG2a, Kappa) for 1 hour, and then treated with C-dextran or PEI (25 μg mL−1) for 6 hours and (C) from WT or TLR-4−/− tumour bearing-mice and treated with C-dextran or PEI (25 μg mL−1) for 6 hours were determined by ELISA; (D–F) Transcriptional levels of iNOS2 and Arg1 in MDSCs from each treatment cohort were analysed by qRT-PCR. The data represent 3–4 independent experiments (n = 3–4, *P ≤ 0.05 and **P ≤ 0.01 versus saline).

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