Figure 2: Effect of the cationic polymers on the immunosuppressive functions of tumour-infiltrating MDSCs. | Scientific Reports

Figure 2: Effect of the cationic polymers on the immunosuppressive functions of tumour-infiltrating MDSCs.

From: Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

Figure 2

The proliferation of CD3+T cells was tested with the CFSE dilution assay, after the labelled splenocytes were co-cultured with the differently treated MDSCs (2:1) for 4 days in the presence of Con A: (A,D) MDSCs from tumour- bearing mice were treated with C-dextran, PEI or dextran (25 μg mL−1) for 6 hours and rinsed before co-culture; (B,E) MDSCs were pre-treated with PBS, TLR-4-neutralising antibody (MTS510) and control IgG for 1 hour, treated with C-dextran or PEI (25 μg mL−1) for 6 hours and rinsed before co-culture; (C,F) MDSCs from WT or TLR-4−/− (KO) tumour-bearing mice were treated with C-dextran or PEI (25 μg mL−1) for 6 hours and rinsed before co-culture. The data represent 3–4 independent experiments (n = 3–4, *P ≤ 0.05 versus saline).

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