Figure 1
From: High Resolution Dissection of Reactive Glial Nets in Alzheimer’s Disease
A broadly accessible, systematic method to study neurons and glia in human brain samples recovered from long-term storage.
(a) The staining of pan-axonal neurofilaments (SMI312) reveals the general organization of neurons in cortical sections (female, 86 years old, 25 years of fixation). (b) Calbindin expressing interneurons are enriched in layers I/II of the human cortex (male, 88 years old, 19 years of fixation) and show diversified morphologies after 3D reconstruction. (c) High-resolution imaging of synapses in cortex (female, 82 years old, 15 years of fixation) shows vGlut1-positive presynaptic boutons (magenta) juxtaposed to PSD95-positive postsynaptic structures (green) (synapses indicated by arrows). (d) Maximum projection of a large field showing 1,95 mm2 (x:1.95 mm, y:0.9 mm) of tissue with a 30 μm depth labeled for Iba1 (microglia; magenta) and GFAP (astrocytes; green). (e) High magnification image of a GFAP-positive astrocyte ‘island’ in a cortical section. (f) 3-dimensional reconstructions of cortical protoplasmic GFAP+ astrocytes and Iba1+ microglia (female, 86 years old). (g) GFAP+ astrocytes (green) and Iba1+ microglia (magenta) in cortical layers III/IV show complex interactions in a control brain (female, 86 years old), resolvable by visualizing individual frames of confocal Z-stacks (step-size: 1 μm). Scale bars: 50 μm (a); 20 μm (b); 5 μm (c); 200 μm (d); 10 μm (e–g).
