Figure 6
From: High Resolution Dissection of Reactive Glial Nets in Alzheimer’s Disease
Human RGNs are associated with inflammation in AD.
(a–c) RGN astrocytes in AD tissue express the pro-inflammatory cytokines IL-6 and IL-1β and the IL-1β processing enzyme Caspase 1. (a) 3D projection showing IL-6-expressing GFAP+ astrocytes near an Aβ plaque in AD cortex (male, 87 years old). Overlay shows IL-6 (magenta), GFAP (green) and Thiazine red (cyan). (b) 3D projection showing IL-1β expression (magenta) in GFAP+ astrocytes (green) around an Thiazine red-labeled Aβ plaque (cyan). (c) Reactive astrocytes (magenta) close to Aβ plaques (blue; dotted ellipse) also express the IL-1β processing enzyme Caspase 1 (green; arrowhead) in the same AD patient. (d,e) Astrocyte are sources of pro-inflammatory cytokines inside and outside RGNs: Examples of astrocytic IL-6 (d, magenta) and IL-1β (e, magenta) expression inside and outside RGNs with the local astrocyte network (GFAP+, green) in AD cortex. Scale bars: 20 μm (a,b,d,e), 10 μm (c).
