Table 2 Chart summarizing antibodies used for immunolabeling procedures.
From: High Resolution Dissection of Reactive Glial Nets in Alzheimer’s Disease
Target | Manufacturer | Reference number | Host species | Mouse tissue IF | Human tissue IF | WB Mouse |
|---|---|---|---|---|---|---|
GFAP | SYnaptic SYstems | 173 004 | Guinea Pig | 1/500 | 1/500 | – |
GFAP | Millipore | MAB360 | Mouse | 1/500 | NW | 1/2000 |
GFAP | Dako | z0334 | Rabbit | 1/1000 | 1/1000 | – |
Iba1 | Wako | 1919741 | Rabbit | 1/500 | 1/500 | – |
Iba1 | Abcam | ab107159 | Goat | 1/500 | NW | – |
Glutamine synthetase | Millipore | MAB302 | Mouse | 1/500 | NW | – |
MHCII | Abcam | ab8351 | Mouse | – | – | 1/5000 |
PSD95 | Cell Signaling | 75–066 | Rabbit | – | 1/250* | – |
GAPDH | Abcam | ab9484 | Mouse | – | – | 1/50000 |
SMI312 | Covance | SMI-312R | Mouse | 1/250 | 1/250* | – |
Calbindin D-28k | SYnaptic SYstems | 214004 | Guinea pig | – | 1/250* | – |
VGLUT1 | NeuroMab | 75–066 | Mouse | – | 1/200* | – |
IL-6 | Biosource | ARC0062 | Rabbit | 1/200 | – | 1/4000 |
IL-6 | Millipore | CBL2117 | Mouse | – | 1/200 | – |
IL-1β | Abcam | ab9722 | Rabbit | 1/200 | – | – |
IL-1β (mature form) | Abcam | ab2105 | Rabbit | – | 1/200 | – |
IL-1β | Rockland | 210-401-319 | Mouse | – | 1/200 | 1/2000 |
PS422 | Invitrogen | 44-764G | Rabbit | 1/250 | 1/250 | – |
AT8 | Thermo Scientific | MN1020 | Mouse | 1/250 | 1/250 | – |
