Figure 1

Effects of naringin on the protection of the trabecular microstructure as analysed by Micro-CT scanning.

The region of interest (ROI) that was chosen for analysis was a region 25–125 slices away from the distal femoral growth plate at the distal femoral metaphysis. The thickness of a slice was 21 μm. (A) Representative 3D images showing the trabecular microarchitecture in the distal femoral metaphysis of each group. (B) Micro-structure parameters of the distal femoral trabecula as analysed from Micro-CT scanning data. Naringin at higher doses prevents the femoral trabecular micro-structure from deteriorating by immobilization at 4 weeks. L represents the 30 mg/kg Naringin group, M the 100 mg/kg Naringin group and H the 300 mg/kg Naringin group. (a) represents the trabecular thickness (Tb.Th), (b) the trabecular number (Tb.N), (c) the trabecular bone volume fraction (BV/TV), (d) the trabecular separation (Tb.Sp) and (e) the surface/volume ratios (BS/BV). ^ap < 0.05, ^aap < 0.01, ^aaap < 0.001 vs. Sham group. ^bp < 0.05, ^bbp < 0.01, ^bbbp < 0.001 vs. USN group. ^cp < 0.05, ^ccp < 0.01, ^cccp < 0.001 vs. 300 mg/kg Naringin group. One-way ANOVA.