Figure 4
Effect of naringin on osteogenesis and osteoclastogenesis in vivo in each group.
The osteoblastic activities in sections of the distal femoral metaphysis taken 4 weeks post-operation were indicated by the immunohistochemical staining of OCN (osteocalcin) and osteoclasts in the distal femoral metaphysis were indicated by TRAP staining. The immunohistochemical reactivity of OCN in the USN group was lower than that of the Sham group, while the OCN staining in the groups that were administered naringin was gradually enhanced. More TRAP-positive cells were observed in the USN group compared to the Sham group; this number significantly decreased in the naringin-treated groups at higher doses. Osteoclasts were indicated as TRAP-positive cells containing >2 nuclei and stained red on the surface of the trabecular bone. L represents the 30 mg/kg Naringin group, M the 100 mg/kg Naringin group and H the 300 mg/kg Naringin group.
