Figure 5

Effect of naringin on the differentiation of osteoclasts and osteoblasts in vitro.

The differentiation of RAW 264.7 cells into osteoclasts was assessed by TRAP staining 7 days after naringin treatment. The differentiation of MC3T3-E1 cells into osteoblasts was evaluated using an ALP activity kit 7 days after naringin treatment. The dose of 20 ng/ml naringin dramatically decreased the number of TRAP-positive osteoclasts (a,b) and increased the ALP activities of osteoblasts in a dose-dependent manner; 20 ng/ml naringin showed the maximum effect (c). The experiments were performed in triplicate, n = 3 per experiment. ^ap < 0.05, ^aap < 0.01, ^aaap < 0.001 vs. Sham group. ^bp < 0.05, ^bbp < 0.01, ^bbbp < 0.001 vs. USN group. ^cp < 0.05, ^ccp < 0.01, ^cccp < 0.001 vs. 300 mg/kg Naringin group. Student’s t test for (b), One-way ANOVA for (c).